31. In the above problem, colonies that were white in the presence of X-gal contained
32. HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following
I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine.
II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.
I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine.
II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.
33. Which among the following immunological techniques is based on passive diffusion of antigen and antibody towards each other
34. Alpha and Beta amylase help in
35. Embryonic stem cells are derived from
36. Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because
37. Somatic cell gene transfer is used for
P. Transgenic animal production.
Q. Transgenic diploid cell production.
R. In vitro fertilization.
S. Classical breeding of farm animals.
P. Transgenic animal production.
Q. Transgenic diploid cell production.
R. In vitro fertilization.
S. Classical breeding of farm animals.