Animal Biotechnology MCQ Questions & Answers | Biotechnology Page-5 section-1

41.
Bacillus subtilis under ideal conditions occupies 5% of the total volume of a fermenter. Under such conditions; the culture medium (after bacilli are filtered off) has a concentration of 1mg/ml of the secreted protein trehalae
(i) Calculate the volume of culture filtrate required to purify 1 kg of trehalase
(ii) What are the total number of B. subtilis cells which produce this amount of enzyme? Assume that each cell is a cylinder which has dimensions of 1 m diameter and length 2μm.

A. (i) 10⁶ ml, (ii) 1.57 × 10⁸

B. (i) 10⁸ ml, (ii) 3.16 × 10¹⁸

C. (i) 10⁶ ml, (ii) 1.57 × 10⁸

D. (i) 10⁶ ml, (ii) 3.18 × 10¹⁶

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42.
In animal cell cultures, the addition of serum to media is essential for providing

A. Amino acids for protein synthesis

B. Nucleotides for DNA synthesis

C. Growth factors

D. All of the above

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43.
A knockout mouse is so named because it contains

A. Aggression genes

B. Multiple copies of a human gene

C. An activated human gene

D. None of the above

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44.
In animal cell culture, a CO₂ enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is correct?

A. Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO₂

B. Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO₂

C. Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO₂

D. CO₂ requirement is independent of bicarbonate concentration in the medium

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45.
Which of these would not Be-used to introduce DNA in animal cells?

A. Liposomes

B. Electroporation

C. Microinjection

D. Ti-plasrnid

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46.
The first patent for a transgenic organism was awarded in 1988. Which organism was patented?

A. A yeast used in industrial processes

B. A bacterium able to metabolize components of crude oil

C. A mouse that manufactures human protein in its milk

D. Life forms cannot be patented

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47.
Carbon-dioxide when combined with the amino groups in haemoglobin molecule and forms

A. HbCO₂

B. HbCO

C. Hb(CO)(-)₃

D. Hb(CO₃)₂

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48.
Term hybridoma implies

A. DNA-RNA hybrid

B. Recombination of DNA molecules

C. Somatic hybridisation

D. Gametic fusion

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49.
DNA ligase of E. coli requires which of the following cofactors?

A. Flavin-adenine dinucleotide as an electron acceptor

B. Nicotinamide-adenine dinucleotide phosphate as a phosphate donor

C. Nicotinamide-adenine dinucleotide (NAD⁺) to form an active adenyl enzyme

D. (NAD⁺) as an electron acceptor

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50.
The syndrom es in human in which an individual's somatic cells contain only the one sex chromosome XO is called

A. Klinefelter syndrome

B. Turner syndrome

C. Downs syndrome

D. Super female

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Animal Biotechnology MCQ Questions & Answers | Biotechnology

Answer & Solution

42. In animal cell cultures, the addition of serum to media is essential for providing

Answer & Solution

43. A knockout mouse is so named because it contains

Answer & Solution

44. In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is correct?

Answer & Solution

45. Which of these would not Be-used to introduce DNA in animal cells?

Answer & Solution

46. The first patent for a transgenic organism was awarded in 1988. Which organism was patented?

Answer & Solution

47. Carbon-dioxide when combined with the amino groups in haemoglobin molecule and forms

Answer & Solution

48. Term hybridoma implies

Answer & Solution

49. DNA ligase of E. coli requires which of the following cofactors?

Answer & Solution

50. The syndrom es in human in which an individual's somatic cells contain only the one sex chromosome XO is called

Answer & Solution

42.
In animal cell cultures, the addition of serum to media is essential for providing

43.
A knockout mouse is so named because it contains

44.
In animal cell culture, a CO₂ enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is correct?

45.
Which of these would not Be-used to introduce DNA in animal cells?

46.
The first patent for a transgenic organism was awarded in 1988. Which organism was patented?

47.
Carbon-dioxide when combined with the amino groups in haemoglobin molecule and forms

48.
Term hybridoma implies

49.
DNA ligase of E. coli requires which of the following cofactors?

50.
The syndrom es in human in which an individual's somatic cells contain only the one sex chromosome XO is called