Animal Biotechnology MCQ Questions & Answers | Biotechnology Page-8 section-1

71.
Methotrexate (MTX) resistant cell lines developed through selection show

A. Increased MYX uptake

B. Reduced production of DHFR

C. Changed DHFR with reduced affinity for MTX

D. None of the above

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72.
Hybridoma technology is used to produce

A. Monoclonal antibodies

B. Polyclonal antibodies

C. Both monoclonal and polyclonal antibodies

D. B cells

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73.
Match the following reporter gene with their source

Column-I Column-II

A. β-Galactosidase 1. Firefly

B. Lux 2. E. coli

C. TK 3. Herpers simplex virus

D. Blasticidin deaminase (bed) 4. Aspergillus terrcus

A. A-3, B-2, C-1, D-4

B. A-2, B-1, C-3, D-4

C. A-3, B-1, C-2, D-4

D. A-2, B-4, C-3, D-1

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74.
For the growth of T-cell, the growth factor needed would be

A. Epidermal growth factor

B. Interleukin-2

C. Fibroblast growth factor

D. TNF-α

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75.
A hybrid derived from the fusion of a myeloma cell (HPRT⁻) with an antibody secreting B-lymphocyte (HPRT⁺) can be selected to produce monoclonal antibody by growing in a medium containing

A. Thiamine, Hypoxanthine, Aminopterin

B. Thymidine, Histidine, Aminopterin

C. Uridine, Hypoxanthine, Aminopterin

D. Thymidine, Hypoxanthine, Aminopterin

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76.
A bacterial culture contains 108 cells/ml in the beginning. Two hours later, it was found to contain 10¹² cells/ml. Calculate the doubling time.

A. 0.30 hr

B. 0.15 hr

C. 0.24 hr

D. 0.50 hr

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77.
r-HuEPO is

A. Human genome

B. Hybridoma technology

C. Recombinant Erythropoietin

D. None of these

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78.
Receptor R is over expressed in CHO cells and analyzed for expression. 6 × 10⁷ cells were incubated with its radioactive ligand (specific activity 100 counts per picomole). If the total counts present in cell pellet was 1000 cpm. the average number of receptors R per cell is (assume complete saturation of receptors with ligand and one ligand binds to one receptor)

A. 10⁴

B. 10⁵

C. 10⁶

D. 10⁷

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79.
Which one of the following is NOT used for the measurement of cell viability in animal cell culture?

A. Trypan blue dye exclusion

B. Tetrazolium (MTT) assay

C. LDH activity in the culture medium

D. Coulter counter

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80.
Gene therapy that is hereditary relates to

A. Somatic cells

B. Germline cells

C. Cancer cells

D. Udder cells

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Column-I	Column-II
A. β-Galactosidase	1. Firefly
B. Lux	2. E. coli
C. TK	3. Herpers simplex virus
D. Blasticidin deaminase (bed)	4. Aspergillus terrcus

Animal Biotechnology MCQ Questions & Answers | Biotechnology

71. Methotrexate (MTX) resistant cell lines developed through selection show

Answer & Solution

72. Hybridoma technology is used to produce

Answer & Solution

73. Match the following reporter gene with their source Column-I Column-II A. β-Galactosidase 1. Firefly B. Lux 2. E. coli C. TK 3. Herpers simplex virus D. Blasticidin deaminase (bed) 4. Aspergillus terrcus

Answer & Solution

74. For the growth of T-cell, the growth factor needed would be

Answer & Solution

75. A hybrid derived from the fusion of a myeloma cell (HPRT−) with an antibody secreting B-lymphocyte (HPRT+) can be selected to produce monoclonal antibody by growing in a medium containing

Answer & Solution

76. A bacterial culture contains 108 cells/ml in the beginning. Two hours later, it was found to contain 1012 cells/ml. Calculate the doubling time.

Answer & Solution

77. r-HuEPO is

Answer & Solution

Answer & Solution

79. Which one of the following is NOT used for the measurement of cell viability in animal cell culture?

Answer & Solution

80. Gene therapy that is hereditary relates to

Answer & Solution

71.
Methotrexate (MTX) resistant cell lines developed through selection show

72.
Hybridoma technology is used to produce

73.
Match the following reporter gene with their source

Column-I Column-II

A. β-Galactosidase 1. Firefly

B. Lux 2. E. coli

C. TK 3. Herpers simplex virus

D. Blasticidin deaminase (bed) 4. Aspergillus terrcus

74.
For the growth of T-cell, the growth factor needed would be

75.
A hybrid derived from the fusion of a myeloma cell (HPRT⁻) with an antibody secreting B-lymphocyte (HPRT⁺) can be selected to produce monoclonal antibody by growing in a medium containing

76.
A bacterial culture contains 108 cells/ml in the beginning. Two hours later, it was found to contain 10¹² cells/ml. Calculate the doubling time.

77.
r-HuEPO is

79.
Which one of the following is NOT used for the measurement of cell viability in animal cell culture?

80.
Gene therapy that is hereditary relates to