13.
Histones have very high percentage of basic amino acids (15 - 30%). For this class of proteins which of the following reagents would be a suitable choice for generating peptides in determination of amino acid sequence of the protein

16.
Order the following events observed during muscle contraction in the correct sequence
1. The sarcomere shortens
2. ATP is hydrolyzed
3. Ca2+ binds to troponin
4. ATP binds to myosin
5. Mosin contracts F-actin
6. F-actin moves
7. Tropomyosin moves
8. Ca2+ is released from the sarcoplasmic reticulum

17.
Under standard temperature (T) and pressure (P) conditions, 128 g of an ideal gas molecule A occupies a volume of 1 L. The gas molecule A obeys the relationship RT = 0.25 PV. R and V are universal gas constant and ideal gas volume, respectively. The molecule A is

19.
Which of the following statements are correct? Very long DNA fragments (> 100 kb) from eukaryotic genomes
P. Can be efficiency packaged in a λ phage vector
Q. Can be propagated in yeast artificial chromosomes
R. Must have cohesive ends for cloning
S. Can be separated by pulsed field gel electrophoresis techniques

20.
Which one of the following statements about eukaryotic RNA polymerases I, II and III is false?