21. Choose the correct completion of the following statement. A fermenter sterilization in situ is less efficient than continuous heat sterilization because
22. Mechanism of separation of contaminants present in air by fibrous media are
23. Match the entries in the Group I with the elution conditions in Group II.
Group I
Group II
P. Ion exchange chromatography
1. Isocratic solvent
Q. Hydrophobic column chromatography
2. Ampholytes
R. Gel filtration chromatography
3. Increasing gradient of salt
S. Chromatofocusing
4. Decreasing gradient of polarity
| Group I | Group II |
| P. Ion exchange chromatography | 1. Isocratic solvent |
| Q. Hydrophobic column chromatography | 2. Ampholytes |
| R. Gel filtration chromatography | 3. Increasing gradient of salt |
| S. Chromatofocusing | 4. Decreasing gradient of polarity |
24. Which of the following cases are likely to lead to faster rates of catalysis by an enzyme immobilized on a negatively charged support?
25. The Pasteur Effect is
26. Fed batch cultivation is suitable for which of the following?
P. Processes with substrate inhibition
Q. Processes with product inhibition
R. High cell density cultivation
P. Processes with substrate inhibition
Q. Processes with product inhibition
R. High cell density cultivation
27. Ultrafiltration process cannot be used for
28. For scaling up for a bioreactor, the following parameter is assumed to be constant
29. Which of the following events occur during the stationary phase of bacterial growth?
P. Rise in cell number stops.
Q. Spore formation in some Gram-positive bacteria such as Bacillus subtilis.
R. Cell size increases in some Gram-negative bacteria such as Escherichia coli.
S. Growth rate of bacterial cells nearly equals their death rate.
T. Decrease in peptidoglycan cross-linking.
P. Rise in cell number stops.
Q. Spore formation in some Gram-positive bacteria such as Bacillus subtilis.
R. Cell size increases in some Gram-negative bacteria such as Escherichia coli.
S. Growth rate of bacterial cells nearly equals their death rate.
T. Decrease in peptidoglycan cross-linking.