61. In chemostat operating under steady state, a bacterial culture can be grown at dilution rate higher than maximum growth rate by
62. Substrate consumption in lag phase of microbial growth is primarily used for
P. Turnover of the cell material.
Q. Maintenance of intracellular pH.
R. Motility.
S. Increase in cell number.
P. Turnover of the cell material.
Q. Maintenance of intracellular pH.
R. Motility.
S. Increase in cell number.
63. Cibacron Blue dye affinity chromatography can be used for affinity purification of
64. A culture vessel in which physical, physicochemical and physiological conditions, as well as cell concentration, are kept constant is known as
65. Inversion of sucrose by immobilized invertase follows a substrate inhibited kinetics. The reaction rate (v) in mol m-3 h-1 can be expressed as:
$$v = \frac{{800[S]}}{{\left\{ {400 + 50[S] + {{[S]}^2}} \right\}}};$$ where [S] is the sucrose concentration.
The immobilized invertase preparation is used in a CSTR with 100 mol m-3 sucrose concentration in the feed stream. If the reaction velocity passes through a maximum at [S] = 20 mol m-3 the feed flow rate for a reactor volume of 1 m3 to get the maximum productivity from the reactor should be
$$v = \frac{{800[S]}}{{\left\{ {400 + 50[S] + {{[S]}^2}} \right\}}};$$ where [S] is the sucrose concentration.
The immobilized invertase preparation is used in a CSTR with 100 mol m-3 sucrose concentration in the feed stream. If the reaction velocity passes through a maximum at [S] = 20 mol m-3 the feed flow rate for a reactor volume of 1 m3 to get the maximum productivity from the reactor should be
66. A bioreactor is scaled up based on equal impeller tip speed. Consider the following parameters for small and large bioreactors:
Parameters
Small bioreactor
Large bioreactor
Impeller speed
N1
N2
Diameter of impeller
D1
D2
Power consumption
P1
P2
Assuming geometrical similarity and the bioreactors are operated in turbulent regime, what will be $$\frac{{{P_2}}}{{{P_1}}}$$ ?
| Parameters | Small bioreactor | Large bioreactor |
| Impeller speed | N1 | N2 |
| Diameter of impeller | D1 | D2 |
| Power consumption | P1 | P2 |
Assuming geometrical similarity and the bioreactors are operated in turbulent regime, what will be $$\frac{{{P_2}}}{{{P_1}}}$$ ?
67. How many kilograms of ethanol is produced from 1 kilogram of glucose in ethanol fermentation?
68. Match the bioreactor components in Group I with the most appropriate function given in Group II.
Group I
Group II
P. Marine type impeller
1. Recirculation of medium
Q. Draft tube
2. Aeration of medium
R. Diaphragm valve
3. Animal cell cultivation
S. Sparger
4. Sterile operation
| Group I | Group II |
| P. Marine type impeller | 1. Recirculation of medium |
| Q. Draft tube | 2. Aeration of medium |
| R. Diaphragm valve | 3. Animal cell cultivation |
| S. Sparger | 4. Sterile operation |
69. Immobilization of enzymes
P. Increases the specificity of the enzyme for its reactants.
Q. Facilitates reuse of the enzyme in batch reactions.
R. Makes it unsuitable for its use in a continuous reactor system.
S. Decreases the operational cost of the industrial process.
P. Increases the specificity of the enzyme for its reactants.
Q. Facilitates reuse of the enzyme in batch reactions.
R. Makes it unsuitable for its use in a continuous reactor system.
S. Decreases the operational cost of the industrial process.