81. An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than one could be apparently due to one of the following reasons. Identify the correct reason.
82. If the dissociation constant for solute-adsorbent binding is KD, the retention time of the solute in a chromatography column
83. In large scale fermentation process, air is sterilized by
84. In large-scale fermentation, the preferred method of sterilization is
85. Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm-1. At a concentration of 50 mg L-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:
86. Match the entries in Group I with the process parameters in Group II.
Group I
Group II
P. Clark electrode
1. Liquid level
Q. Redox probe
2. Dissolved oxygen concentration
R. Load cell
3. Vessel pressure
S. Diaphragm gauge
4. pH (anaerobic process)
| Group I | Group II |
| P. Clark electrode | 1. Liquid level |
| Q. Redox probe | 2. Dissolved oxygen concentration |
| R. Load cell | 3. Vessel pressure |
| S. Diaphragm gauge | 4. pH (anaerobic process) |