21.
Identify the character-based method(s) used for the construction of a phylogenetic tree.
P. Maximum parsimony
Q. Neighbor joining
R. Maximum likelihood
S. Bootstrapping

23.
Amino acid sequences of cytochrome c and ribulose 5-phosphate epimerase from 40 organisms were chosen and phylogenetic trees were obtained for each of these two protein families.
Determine the correctness or otherwise of the following Assertion [A] and the Reason [R].
Assertion [A]: The two trees will not be identical.
Reason [R]: The nature and frequency of mutations in the two families are different.

24.
Match the items in Group I with an appropriate description in Group II.
Group I Group II
P. UPGMA 1. Protein sequence database
Q. CLUSTAL 2. Phylogenetic analysis
R. SWISS-PROT 3. 3-D structure visualization
S. RasMol 4. Multiple sequence alignment