11. Enzyme used in 'cycle' sequencing of DNA is
12. Which one of the following is not a requirement for a PCR reaction?
13. Which of the following statements about gene therapy are correct?
P. Affected individuals, but not their progeny, can be cured through germline gene therapy.
Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy.
R. Affected individuals, but not their progeny, can be cured through somatic gene therapy.
S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.
P. Affected individuals, but not their progeny, can be cured through germline gene therapy.
Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy.
R. Affected individuals, but not their progeny, can be cured through somatic gene therapy.
S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.
14. Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction?
P. Mg2+ concentration
Q. pH
R. Annealing temperature
P. Mg2+ concentration
Q. pH
R. Annealing temperature
15. The heterozygosity of any locus can be ascertained by
16. For PCR to amplify the sequence
ATCTTCTACG . . . . . . . . AAGCPTGCGG
TAGAAGATGC . . . . . . . .TTCGAACGCC
The required primers are
ATCTTCTACG . . . . . . . . AAGCPTGCGG
TAGAAGATGC . . . . . . . .TTCGAACGCC
The required primers are
17. In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be
18. In human populations 4% of the individuals are homozygous recessive to a specific RFLP marker. What percentage of individuals does you expect to be heterozygous for this trait?
19. Which of the following fluorescent probes is used to monitor the progress of amplification in Real time PCR?
20. Match items in Group I with correct options from those given in Group II.
Group I
Group II
P. VNTR sequence
1. Gene regulation on the same chromosome
Q. Leader sequence
2. Ribosome binding site
R. SD sequence
3. DNA fingerprinting
S. cis-acting sequence
4. Functions in attenuation
| Group I | Group II |
| P. VNTR sequence | 1. Gene regulation on the same chromosome |
| Q. Leader sequence | 2. Ribosome binding site |
| R. SD sequence | 3. DNA fingerprinting |
| S. cis-acting sequence | 4. Functions in attenuation |