21. For the sequence of dsDNA given below, identify the set of primers required to amplify this DNA by PCR.
3' GACTCCA . . . . . . . . TACAACC 5'
5' CTGAGGT . . . . . . . . ATGTTGG 3'
3' GACTCCA . . . . . . . . TACAACC 5'
5' CTGAGGT . . . . . . . . ATGTTGG 3'
22. Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR).
5'-GACCTGTGG . . . . . . . . ATACGGGAT-3'
3'-CTGGACACC . . . . . . . . TATGCCCTA-5'
Primers
P. 5'-GACCTGTGG-3'
Q. 5'-CCACAGGTC-3'
R. 5'-TAGGGCATA-3'
S. 5'-ATCCCGTAT-3'
5'-GACCTGTGG . . . . . . . . ATACGGGAT-3'
3'-CTGGACACC . . . . . . . . TATGCCCTA-5'
Primers
P. 5'-GACCTGTGG-3'
Q. 5'-CCACAGGTC-3'
R. 5'-TAGGGCATA-3'
S. 5'-ATCCCGTAT-3'
23. Protein binding regions of DNA are identified by one of the following techniques.
24. Nick translation of DNA is a method for making DNA probes. Identify from below what is not required for nick translation method.
25. Match the reagents in Group I with their preferred cleavage sites in Group II.
Group I
Group II
P. Cyanogen bromide
1. Carboxyl side of methionine
Q. o-Iodosobenzoate
2. Amino side of methionine
R. Hydroxylamine
3. Carboxyl side of tryptophan
S. 2-Nitro-5-thiocyanobenzoate
4. Amino side of cysteine
5. Asparagine-glycine bonds
Group I | Group II |
P. Cyanogen bromide | 1. Carboxyl side of methionine |
Q. o-Iodosobenzoate | 2. Amino side of methionine |
R. Hydroxylamine | 3. Carboxyl side of tryptophan |
S. 2-Nitro-5-thiocyanobenzoate | 4. Amino side of cysteine |
5. Asparagine-glycine bonds |