31. Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially?
32. CRISPR-Cas system is associated with
33. Identify the correct statements.
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction.
Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid.
R. T4 polynucleotide kinase is used for labeling 3' end of DNA.
S. Baculovirus (Autographa californica) can be used as an insect expression vector.
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction.
Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid.
R. T4 polynucleotide kinase is used for labeling 3' end of DNA.
S. Baculovirus (Autographa californica) can be used as an insect expression vector.
34. W, X and Y are the intermediates in a biochemical pathway as shown below:
$$S \cdot \cdot \cdot \cdot \cdot \cdot \cdot \cdot \to W \to X \to Y \to Z$$
Mutants auxotrophic for Z are found in four different complementation groups, namely Z1, Z2, Z3 and Z4. The growth of these mutants on media supplemented with W, X, Y or Z is shown below (Yes: growth observed: No: growth not observed):
Mutants
Media Supplemented with Mutants
W
X
Y
Z
Z1
No
No
Yes
Yes
Z2
No
Yes
Yes
Yes
Z3
No
No
No
Yes
Z4
Yes
Yes
Yes
Yes
What is the order of the four complementation groups in terms of the step they block?
$$S \cdot \cdot \cdot \cdot \cdot \cdot \cdot \cdot \to W \to X \to Y \to Z$$
Mutants auxotrophic for Z are found in four different complementation groups, namely Z1, Z2, Z3 and Z4. The growth of these mutants on media supplemented with W, X, Y or Z is shown below (Yes: growth observed: No: growth not observed):
| Mutants | Media Supplemented with Mutants | |||
| W | X | Y | Z | |
| Z1 | No | No | Yes | Yes |
| Z2 | No | Yes | Yes | Yes |
| Z3 | No | No | No | Yes |
| Z4 | Yes | Yes | Yes | Yes |
What is the order of the four complementation groups in terms of the step they block?
35. For amplification of GC rich sequences by polymerase chain reaction, identify the reagent that hinds and stabilizes AT sequences and destabilizes GC regions.
36. In order to identify the person who committed a crime, forensic experts will need to extract DNA from the tissue sample collected at the crime scene, and conduct one of the following procedures for DNA fingerprinting analysis
37. Restriction endonucleases from two different organisms that recognize the same DNA sequence for cleavage are called
38. Protein-DNA interactions in vivo can be studied by
39. The DNA sequence shown below is to be amplified by PCR:
5' GCTAAGATCTGAATTTTCC . . . . . . . .
TTGGGCAATAATGTAGCGC 3'
3' CGATTCTAGACTTAAAAGG . . . . . . . .
AACCCGTTATTACATCGCG 5'
Which one of the following pair of primers can be used for this amplification?
5' GCTAAGATCTGAATTTTCC . . . . . . . .
TTGGGCAATAATGTAGCGC 3'
3' CGATTCTAGACTTAAAAGG . . . . . . . .
AACCCGTTATTACATCGCG 5'
Which one of the following pair of primers can be used for this amplification?