11. Phage T7 promoter containing plasmids are used for over-expression of cloned genes because
12. Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because
13. The restriction endonuclease Eco52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco52I?
14. Which of the following are commonly used as reporter genes?
P. NPTgene
Q. Luciferase gene
R. CFTR gene
S. GFP gene
P. NPTgene
Q. Luciferase gene
R. CFTR gene
S. GFP gene
15. Which one of the following statements about Agrobacterium Ti plasmid is correct?
16. Match the items in Group I with Group II.
Group I (Vectors)
Group II (Maximum DNA packaging)
P. λ phage
1. 35 - 45 kb
Q. Bacterial Artificial Chromosomes (BACs)
2. 100 - 300 kb
R. P1 derived Artificial Chromosomes (PACs)
3. ≤ 300 kb
S. λ cosmid
4. 5 - 25 kb
| Group I (Vectors) | Group II (Maximum DNA packaging) |
| P. λ phage | 1. 35 - 45 kb |
| Q. Bacterial Artificial Chromosomes (BACs) | 2. 100 - 300 kb |
| R. P1 derived Artificial Chromosomes (PACs) | 3. ≤ 300 kb |
| S. λ cosmid | 4. 5 - 25 kb |
17. A single base pair of DNA weighs 1.1 × 10-21 grams. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 μg of purified DNA?
18. Many plasmids have ampr marker. This implies
19. Expression in poor amount and in inactive form of cDNA of a eukaryotic protein in Escherichia coli using its expression vector is due to
P. The absence of capping mechanism of mRNA.
Q. Codon bias.
R. Absence of polyadenylation.
S. Absence of proper glycosylation.
P. The absence of capping mechanism of mRNA.
Q. Codon bias.
R. Absence of polyadenylation.
S. Absence of proper glycosylation.