21. Restriction endonucleases which recognize and cut same recognition sequences are known as
22. The length of each boarder sequence in Ti plasmid is about
23. A DNA fragment of 4500 bp has to be tailed with dT residues by using dTTP and the enzyme 'terminal transferase' The stock solution of dTTP that is used as a substrate has concentration of 150 μM. Ten μL of this stock solution is added to a total volume of 200 μL reaction. What will be the concentration of dTTP in the reaction?
24. Baculovirus expression system is used to express heterologous genes in
25. A variety of genetic elements are used in the transgenic plant research. Match the genetic elements (Group I) with their corresponding source (Group II).
Group I
Group II
P. Ubiquitin1 promoter
1. Agrobacterium tumefaciens
Q. Nos transcriptional terminator
2. Streptomyces hygroscopicus
R. bar selection marker gene
3. Escherichia coli
S. gus reporter gene
4. Zea mays
| Group I | Group II |
| P. Ubiquitin1 promoter | 1. Agrobacterium tumefaciens |
| Q. Nos transcriptional terminator | 2. Streptomyces hygroscopicus |
| R. bar selection marker gene | 3. Escherichia coli |
| S. gus reporter gene | 4. Zea mays |