Recombinant DNA Technology MCQ Questions & Answers

31.
A gene cannot be isolated from a human genomic DNA library by functional complementation in E. coli because of

A. Non-functional promoter

B. The absence of splicing machinery

C. Coupled transcription and translation

D. Codon bias

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32.
During a positive-negative selection process, transformed animal cells expressing . . . . . . . . are killed in presence of ganciclovir in the medium.

A. Pyruvate kinase

B. Viral thymidine kinase

C. Viral serine/threonine kinase

D. Viral tyrosine kinase

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33.
To isolate a gene coding for glucagon, the cDNA library as to be constructed using mRNA isolated from

A. Intestine

B. Pancreas

C. Pituitary

D. Brain

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34.
Which one of the following cannot be a recognition sequence for a type II restriction enzyme?

A. GAATTC

B. AGCT

C. GCGGCCGC

D. ATGCCT

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35.
To be a cloning vector, a plasmid does not require

A. An origin of replication

B. An antibiotic resistance marker

C. A restriction site

D. To have a high copy number

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36.
Select the correct combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.

A. Border repeat sequences and oncogenes

B. Border repeat sequences and vir genes

C. Opine biosynthetic genes and vir genes

D. Opine biosynthetic genes and oncogenes

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37.
A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?

A. 4 labeled; 6 unlabeled

B. 4 labeled; 4 unlabeled

C. 3 labeled; 5 unlabeled

D. 3 labeled; 3 unlabeled

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38.
A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?

A. 4.50 × 10⁵

B. 3.50 × 10⁵

C. 4.20 × 10⁵

D. 4.05 × 10⁵

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39.
The enzymes that can be used in 5' end labeling of DNA are

A. Alkaline phosphatase

B. DNA ligase

C. Terminal transferase

D. Polynucleotide kinase

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40.
The restriction endonuclease HaeIII recognizes the sequence GG^↓CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice

A. SmaII (CC^↓GGG)

B. NotI (GC^↓GGCCGC)

C. SalmIII (GG^↓CC)

D. PstI (CTGCA^↓G)

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Recombinant DNA Technology MCQ Questions & Answers | Biotechnology

31. A gene cannot be isolated from a human genomic DNA library by functional complementation in E. coli because of

Answer & Solution

32. During a positive-negative selection process, transformed animal cells expressing . . . . . . . . are killed in presence of ganciclovir in the medium.

Answer & Solution

33. To isolate a gene coding for glucagon, the cDNA library as to be constructed using mRNA isolated from

Answer & Solution

34. Which one of the following cannot be a recognition sequence for a type II restriction enzyme?

Answer & Solution

35. To be a cloning vector, a plasmid does not require

Answer & Solution

36. Select the correct combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.

Answer & Solution

37. A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?

Answer & Solution

38. A cell has five molecules of a rare mRNA. Each cell contains 4 × 105 mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?

Answer & Solution

39. The enzymes that can be used in 5' end labeling of DNA are

Answer & Solution

40. The restriction endonuclease HaeIII recognizes the sequence GG↓CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice

Answer & Solution

31.
A gene cannot be isolated from a human genomic DNA library by functional complementation in E. coli because of

32.
During a positive-negative selection process, transformed animal cells expressing . . . . . . . . are killed in presence of ganciclovir in the medium.

33.
To isolate a gene coding for glucagon, the cDNA library as to be constructed using mRNA isolated from

34.
Which one of the following cannot be a recognition sequence for a type II restriction enzyme?

35.
To be a cloning vector, a plasmid does not require

36.
Select the correct combination of genetic components that are essential for the transfer of T-DNA segment from Agrobacterium tumefaciens to plant cells.

37.
A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled?

38.
A cell has five molecules of a rare mRNA. Each cell contains 4 × 10⁵ mRNA molecules. How many clones one will need to screen to have 99% probability of finding at least one recombinant cDNA of the rare mRNA, after making cDNA library from such cell?

39.
The enzymes that can be used in 5' end labeling of DNA are

40.
The restriction endonuclease HaeIII recognizes the sequence GG^↓CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice