Hayflick limit of mammalian cells refers to which one of the following?
A. Cells in primary cell culture undergo senescence after 50-60 passages
B. Primary cells cultured in vitro do not cross the limit of cell transformation
C. Cell lines when cultured in vitro have a limit for their surface to volume ratio
D. Malignant cell lines undergo senescence after 50-60 passages
Answer: Option A
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