Rare cutter is
A. Hae III
B. Alu I
C. Pvu II
D. Eco R I
Answer: Option D
Solution (By Examveda Team)
1. Rare cutters are restriction enzymes that recognize longer and less frequent sequences in DNA.- These enzymes cut DNA at specific recognition sites, usually 6 to 8 base pairs long.
2. Eco R I is a rare cutter.
- It recognizes the specific sequence 5'-GAATTC-3', which occurs less frequently in the genome compared to shorter recognition sequences.
3. Rare cutters are useful in genome mapping and cloning.
- They generate larger DNA fragments, making them ideal for analyzing larger genomic regions.
4. Options like Hae III and Alu I are frequent cutters.
- They recognize shorter sequences and cut more frequently, producing smaller fragments.
5. Option D is correct as Eco R I is classified as a rare cutter due to its longer recognition sequence.
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HaeIII is correct answer