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The mordant used in Gram staining is

A. Safranine

B. Crystal violet

C. Iodine

D. Ethanol

Answer: Option C

Solution (By Examveda Team)

The mordant used in Gram staining is Iodine.
The mordant is Gram's Iodine. This binds to the crystal violet making a large complex that adheres to the cell membrane.
Gram's Iodine is allowed to sit for 30 seconds then the decolourizer, 95% ethanol is added.

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