A bacterial culture having a specific oxygen uptake rate of 5 mmol O2 (g DCW)-1 hr-1 is being grown aerobically in a fed-batch bioreactor. The maximum value of the volumetric oxygen transfer coefficient is 0.18 s-1 for the stirred tank bioreactor and the critical dissolved oxygen concentration is 20% of the saturation concentration (8 mg mL-1). The maximum density to which the cells can be grown in the fed-batch process without the growth being limited by oxygen transfer, is approximately
A. 14 g L-1
B. 26 g L-1
C. 32 g L-1
D. 65 g L-1
Answer: Option D
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