A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg2+. Which one of the following is missing in the reaction mixture?
A. Helicase
B. Single-stranded binding proteins
C. Primers
D. Reverse transcriptase
Answer: Option C
Related Questions on Molecular Tools in Biotechnology
Expression of hundreds of different genes in DNA microarray technology is monitored by using
A. Radioactive probe
B. Visible chromogenic probe
C. UV absorbing probe
D. Fluorescent probe
A. Transcriptionally controlled
B. Post-transcriptionally controlled
C. Translationally controlled
D. Post-translationally controlled
Which one of the following techniques/tools is not used for inserting a foreign gene into a cell?
A. DNA microarray
B. Electroporation
C. Gene gun
D. Microinjection
A. Polymerase chain reaction
B. DNA microarray
C. Northern hybridization
D. Southern hybridization
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