Molecular Tools in Biotechnology MCQ Questions & Answers

1.
Expression of hundreds of different genes in DNA microarray technology is monitored by using

A. Radioactive probe

B. Visible chromogenic probe

C. UV absorbing probe

D. Fluorescent probe

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2.
Identical sized RNA transcript is detected by Northern blot analysis of UDP glucuronosyl transferase obtained from human liver and kidney. Microarray analysis of the same samples shows equal spot intensity, whereas Western blot detects a 55 kDa strong band in liver, but a very faint in kidney of same size. The regulation of UDP glucuronosyl transferase is

A. Transcriptionally controlled

B. Post-transcriptionally controlled

C. Translationally controlled

D. Post-translationally controlled

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3.
Which one of the following techniques/tools is not used for inserting a foreign gene into a cell?

A. DNA microarray

B. Electroporation

C. Gene gun

D. Microinjection

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4.
Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment?

A. Polymerase chain reaction

B. DNA microarray

C. Northern hybridization

D. Southern hybridization

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5.
The nucleotide analogue used in DNA sequencing by chain termination method is

A. 1', 3'-dideoxy nucleoside triphosphate

B. 2', 3'-dideoxy nucleoside triphosphate

C. 2', 4'-dideoxy nucleoside triphosphate

D. 2', 5'-dideoxy nucleoside triphosphate

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6.
Expression of which of the following reporter genes do not require addition of specific substrate for detection?

A. Luciferase

B. β-Glucuronidase

C. β-Glucosidase

D. Green fluorescent protein

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7.
What will be the binding status of regulatory proteins in lac operon when concentrations of both lactose and glucose are very low in the culture medium?

A. Only the repressor remains bound to the operator

B. Only the cyclic AMP-Catabolic Activator Protein (cAMP-CAP) complex remains bound to the CAP binding site

C. Neither the repressor nor cAMP-CAP complex remain bound to their respective binding sites

D. Both the repressor and cAMP-CAP complex remain bound to their respective binding sites

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8.
Match the items in Group I with correct options in Group II.

Group I Group II

P. DNA footprinting 1. Protein-protein interaction

Q. Yeast two-hybrid system 2. VNTR

R. DNA fingerprinting 3. DNA binding protein

S. SAGE 4. Transcriptome analysis

A. P-1, Q-2, R-4, S-3

B. P-3, Q-1, R-2, S-4

C. P-3, Q-4, R-1, S-2

D. P-4, Q-2, R-1, S-3

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9.
A thermostable DNA polymerase that can carry out both reverse transcription reaction and polymerization has been isolated form

A. Thermococcus litoralis

B. Thermus aquaticus

C. Thermotoga maritime

D. Thermus thermophilus

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10.
A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg²⁺. Which one of the following is missing in the reaction mixture?

A. Helicase

B. Single-stranded binding proteins

C. Primers

D. Reverse transcriptase

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Group I	Group II
P. DNA footprinting	1. Protein-protein interaction
Q. Yeast two-hybrid system	2. VNTR
R. DNA fingerprinting	3. DNA binding protein
S. SAGE	4. Transcriptome analysis

Molecular Tools in Biotechnology MCQ Questions & Answers | Biotechnology

1. Expression of hundreds of different genes in DNA microarray technology is monitored by using

Answer & Solution

Answer & Solution

3. Which one of the following techniques/tools is not used for inserting a foreign gene into a cell?

Answer & Solution

4. Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment?

Answer & Solution

5. The nucleotide analogue used in DNA sequencing by chain termination method is

Answer & Solution

6. Expression of which of the following reporter genes do not require addition of specific substrate for detection?

Answer & Solution

7. What will be the binding status of regulatory proteins in lac operon when concentrations of both lactose and glucose are very low in the culture medium?

Answer & Solution

8. Match the items in Group I with correct options in Group II. Group I Group II P. DNA footprinting 1. Protein-protein interaction Q. Yeast two-hybrid system 2. VNTR R. DNA fingerprinting 3. DNA binding protein S. SAGE 4. Transcriptome analysis

Answer & Solution

9. A thermostable DNA polymerase that can carry out both reverse transcription reaction and polymerization has been isolated form

Answer & Solution

10. A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg2+. Which one of the following is missing in the reaction mixture?

Answer & Solution

1.
Expression of hundreds of different genes in DNA microarray technology is monitored by using

3.
Which one of the following techniques/tools is not used for inserting a foreign gene into a cell?

4.
Which one of the following techniques can be used to compare the expression of a large number of genes in two biological samples in a single experiment?

5.
The nucleotide analogue used in DNA sequencing by chain termination method is

6.
Expression of which of the following reporter genes do not require addition of specific substrate for detection?

7.
What will be the binding status of regulatory proteins in lac operon when concentrations of both lactose and glucose are very low in the culture medium?

8.
Match the items in Group I with correct options in Group II.

Group I Group II

P. DNA footprinting 1. Protein-protein interaction

Q. Yeast two-hybrid system 2. VNTR

R. DNA fingerprinting 3. DNA binding protein

S. SAGE 4. Transcriptome analysis

9.
A thermostable DNA polymerase that can carry out both reverse transcription reaction and polymerization has been isolated form

10.
A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg²⁺. Which one of the following is missing in the reaction mixture?