A roller bottle culture vessel perfectly cylindrical in shape having inner radius (r) = 10 cm and length (l) = 20 cm was fitted with a spiral film of length (L) = 30 cm and width (W) = 20 cm. If the film can support 105 anchorage dependent cells per cm2, the increase in the surface area after fitting the spiral film and the additional number of cells that can be grown respectively are
A. 1200 cm2 and 12 × 107 cells
B. 600 cm2 and 6 × 107 cells
C. 600 cm2 and 8300 cells
D. 1200 cm2 and 8300 cells
Answer: Option B
This Question Belongs to Biotechnology Engineering >> Bioprocess Engineering And Process Biotechnology
Which of the following techniques is best suited for immobilizing an affinity ligand?
A. Physical adsorption
B. Gel entrapment
C. Crosslinking with a polymer
D. Covalent linkage to a spacer arm
Which one of the following can not be a limiting substrate if Monod's growth kinetics is applicable?
A. Extracellular carbon source
B. Extracellular nitrogen source
C. Dissolved oxygen
D. Intracellular carbon source
A. P-2, Q-3, R-1, S-4
B. P-1, Q-2, R-4, S-3
C. P-3, Q-2, R-4, S-1
D. P-3, Q-4, R-2, S-1
A. 10 h
B. 0.2 h
C. 4.6 h
D. None of the above
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