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1
The first artificial cloning vector is
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Answer: Option A
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2
The ability to create transgenic organisms allows genetic characteristics to be transferred beyond
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Answer: Option D
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3
The amino acid having carboxylic group is
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Answer: Option D
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4
Holiday model describes
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Answer: Option A
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5
The heavy metals which affect the protein structure is
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Answer: Option D
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6
Rare cutter is
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Answer: Option D
Solution:
1. Rare cutters are restriction enzymes that recognize longer and less frequent sequences in DNA.
- These enzymes cut DNA at specific recognition sites, usually 6 to 8 base pairs long.

2. Eco R I is a rare cutter.
- It recognizes the specific sequence 5'-GAATTC-3', which occurs less frequently in the genome compared to shorter recognition sequences.

3. Rare cutters are useful in genome mapping and cloning.
- They generate larger DNA fragments, making them ideal for analyzing larger genomic regions.

4. Options like Hae III and Alu I are frequent cutters.
- They recognize shorter sequences and cut more frequently, producing smaller fragments.

5. Option D is correct as Eco R I is classified as a rare cutter due to its longer recognition sequence.
7
In a . . . . . . . . protocol, bacteria with engineered abilities to detoxify pollutants are intentionally released in an area.
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Answer: Option C
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8
lac z' gene select the recombinant by
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Answer: Option B
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9
During splicing of proteins which part is removed
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Answer: Option D
Solution:
1. Splicing of proteins refers to the process where specific sequences within a protein are removed and the remaining parts are joined together.

2. Inteins are the parts removed during protein splicing.
- They are internal protein sequences that excise themselves from a precursor protein.

3. Exteins are the remaining protein sequences.
- These sequences are joined together to form the functional protein after the removal of inteins.

4. Protein splicing is an autocatalytic process.
- It does not require external enzymes and occurs naturally in some organisms.

5. Option D is correct because inteins are removed during protein splicing, leaving exteins to form the final protein.
10
The process of using living organisms to remove contaminants, pollutants or unwanted substances from soil or water is known as
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Answer: Option D
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