An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than one could be apparently due to one of the following reasons. Identify the correct reason.
A. The enzyme follows substrate inhibited kinetics with internal pore diffusion limitation
B. The enzymes experiences external film diffusion limitation
C. The enzyme follows sigmoidal kinetics
D. The immobilized enzyme is operationally unstable
Answer: Option A
Which of the following techniques is best suited for immobilizing an affinity ligand?
A. Physical adsorption
B. Gel entrapment
C. Crosslinking with a polymer
D. Covalent linkage to a spacer arm
Which one of the following can not be a limiting substrate if Monod's growth kinetics is applicable?
A. Extracellular carbon source
B. Extracellular nitrogen source
C. Dissolved oxygen
D. Intracellular carbon source
A. P-2, Q-3, R-1, S-4
B. P-1, Q-2, R-4, S-3
C. P-3, Q-2, R-4, S-1
D. P-3, Q-4, R-2, S-1
A. 10 h
B. 0.2 h
C. 4.6 h
D. None of the above
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