Evaluate the Michaelis constant for the following lipase catalyzed transesterification reaction for the production of biodiesel.
$${\text{Vegetale oil}} + {\text{Lipase}} + \mathop \rightleftharpoons \limits_{{{\text{k}}1}}^{{{\text{k}}{ - 1}}} {\text{ Oil-lipase complex }}\mathop \to \limits^{{{\text{k}}_1}} {\text{Biodiesel}} + {\text{Glycerol}}$$
where k1 = 3 × 108 M−1 s−1; k−1 = 4 × 104 s−1 and k2 = 2 × 103 s−1
A. 4.2 × 10-3 M
B. 14.0 × 10-4 M
C. 6.4 × 10-6 M
D. .4 × 10-4 M
Answer: Option D
Related Questions on Bioprocess Engineering and Process Biotechnology
Which of the following techniques is best suited for immobilizing an affinity ligand?
A. Physical adsorption
B. Gel entrapment
C. Crosslinking with a polymer
D. Covalent linkage to a spacer arm
Which one of the following can not be a limiting substrate if Monod's growth kinetics is applicable?
A. Extracellular carbon source
B. Extracellular nitrogen source
C. Dissolved oxygen
D. Intracellular carbon source
A. P-2, Q-3, R-1, S-4
B. P-1, Q-2, R-4, S-3
C. P-3, Q-2, R-4, S-1
D. P-3, Q-4, R-2, S-1
A. 10 h
B. 0.2 h
C. 4.6 h
D. None of the above
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