Evaluate the Michaelis constant for the following lipase catalyzed transesterification reaction for the production of biodiesel.
$${\text{Vegetale oil}} + {\text{Lipase}} + \mathop \rightleftharpoons \limits_{{{\text{k}}1}}^{{{\text{k}}{ - 1}}} {\text{ Oil-lipase complex }}\mathop \to \limits^{{{\text{k}}_1}} {\text{Biodiesel}} + {\text{Glycerol}}$$
where k1 = 3 × 108 M−1 s−1; k−1 = 4 × 104 s−1 and k2 = 2 × 103 s−1
A. 4.2 × 10-3 M
B. 14.0 × 10-4 M
C. 6.4 × 10-6 M
D. .4 × 10-4 M
Answer: Option D

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