Examine the data given below in the table on purification of a protein X.
| Step | Volume (mL) | Protein Concentration (mg mL-1) | Enzyme Activity (unit mL-1) |
| Crude cell-free extract | 500 | 12.0 | 5.0 |
| Ammonium sulfate precipitation | 125 | 3.0 | 11.0 |
| Ion-exchange chromatography | 10 | 9.0 | 75.0 |
The yield percent and purification factor, respectively, at the end of the experiment will be approximately
A. 25 and 3
B. 30 and 20
C. 20 and 30
D. 75 and 30
Answer: Option B
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