Examine the data given below in the table on purification of a protein X.
| Step | Volume (mL) | Protein Concentration (mg mL-1) | Enzyme Activity (unit mL-1) |
| Crude cell-free extract | 500 | 12.0 | 5.0 |
| Ammonium sulfate precipitation | 125 | 3.0 | 11.0 |
| Ion-exchange chromatography | 10 | 9.0 | 75.0 |
The yield percent and purification factor, respectively, at the end of the experiment will be approximately
A. 25 and 3
B. 30 and 20
C. 20 and 30
D. 75 and 30
Answer: Option B
This Question Belongs to Biotechnology Engineering >> Microbial Biotechnology
The precursor for penicillin-G biosynthesis during fermentation process is
A. Phenylacetic acid
B. Phenoxyacetic acid
C. Acetic acid
D. None of the above
Which of the following eukaryotic organisms has been proven to be of great industrial importance?
A. Penicillium chrysogenum
B. Saccharomyces cerevisiae
C. Bacillus subtilis
D. Streptomyces griseus
Oils rich in PUFA are not desirable for biodiesel production because
A. They form epoxides in presence of oxygen
B. They do not form epoxides in presence of oxygen
C. They have high ignition temperature
D. They solidify at low temperature
A. P-3, Q-1, R-4, S-2
B. P-3, Q-4, R-1, S-2
C. P-2, Q-3, R-1, S-4
D. P-2, Q-1, R-4, S-3
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