1. The precursor for penicillin-G biosynthesis during fermentation process is
2. Which of the following eukaryotic organisms has been proven to be of great industrial importance?
3. Oils rich in PUFA are not desirable for biodiesel production because
4. Match the products in Group I with their ppossible applications in Group II.
Group I
Group II
P. Erythropoietin
1. Blood clot
Q. Anti-fibrin 99
2. Binding and transport of iron
R. Collagenase
3. Anaemia
S. S. Transferrin
4. Animal cell separation
| Group I | Group II |
| P. Erythropoietin | 1. Blood clot |
| Q. Anti-fibrin 99 | 2. Binding and transport of iron |
| R. Collagenase | 3. Anaemia |
| S. S. Transferrin | 4. Animal cell separation |
5. Match the recombinant products in Group I with their therapeutic applications in Group II.
Group I
Group II
P. Human growth hormone
1. Pituitary dwarfism
Q. Platelet growth factor
2. Chemotherapy induced thrombocytopenia
R. Factor VIII
3. Hemophilia
S. Erythropoietin
4. Anemia associated with chronic renal failure
| Group I | Group II |
| P. Human growth hormone | 1. Pituitary dwarfism |
| Q. Platelet growth factor | 2. Chemotherapy induced thrombocytopenia |
| R. Factor VIII | 3. Hemophilia |
| S. Erythropoietin | 4. Anemia associated with chronic renal failure |
6. Identify the statement that is not correct
7. Examine the data given below in the table on purification of a protein X.
Step
Volume (mL)
Protein Concentration (mg mL-1)
Enzyme Activity (unit mL-1)
Crude cell-free extract
500
12.0
5.0
Ammonium sulfate precipitation
125
3.0
11.0
Ion-exchange chromatography
10
9.0
75.0
The yield percent and purification factor, respectively, at the end of the experiment will be approximately
| Step | Volume (mL) | Protein Concentration (mg mL-1) | Enzyme Activity (unit mL-1) |
| Crude cell-free extract | 500 | 12.0 | 5.0 |
| Ammonium sulfate precipitation | 125 | 3.0 | 11.0 |
| Ion-exchange chromatography | 10 | 9.0 | 75.0 |
The yield percent and purification factor, respectively, at the end of the experiment will be approximately
8. Match the activity spectrum of the following antibiotics.
Group I
Group II
P. Actinomycin D
1. Antifungal
Q. Daunorubicin
2. Antituberculosis
R. Rifamycin
3. Antitumor
S. Griseofulvin
4. Antiprotozoal
| Group I | Group II |
| P. Actinomycin D | 1. Antifungal |
| Q. Daunorubicin | 2. Antituberculosis |
| R. Rifamycin | 3. Antitumor |
| S. Griseofulvin | 4. Antiprotozoal |
9. Determine the correctness or otherwise of the following Assertion (A) and Reason (R).
Assertion: Cell mass yield of a methylotrophic yeast is more on methanol compared to glucose.
Reason: Methanol has a greater degree of reductance compared to glucose.
Assertion: Cell mass yield of a methylotrophic yeast is more on methanol compared to glucose.
Reason: Methanol has a greater degree of reductance compared to glucose.
10. Match the products in Group I with their producer organisms given in Group II.
Group I
Group II
P. Ethanol
1. Streptomyces orientalis
Q. L-Lysine
2. Saccharomyces cerevisiae
R. Biopesticide
3. Corynebacterium glutanicum
S. Vancomycin
4. Bacillus thuringiensis
| Group I | Group II |
| P. Ethanol | 1. Streptomyces orientalis |
| Q. L-Lysine | 2. Saccharomyces cerevisiae |
| R. Biopesticide | 3. Corynebacterium glutanicum |
| S. Vancomycin | 4. Bacillus thuringiensis |