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HAT (hypoxanthine aminopterin and thymidine) is used for selecting the hybridomas...

This question belongs to Biotechnology Engineering Animal Biotechnology
Animal Biotechnology
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HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following
I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine.
II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.

Answer & Solution
Correct Answer: Option C
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