Animal Biotechnology MCQ Questions & Answers

1

What is the primary purpose of neomycin in creating mice with knockouts in gene X?

A) Neomycin selects for the survival of embryonic stem cells (ES) that have incorporated the mutant gene X anywhere in the genome B) Neomycin selects for the survival of ES cell that have incorporated the mutant gene in the place of the wild-type gene C) Neomycin prevents Candida infection during ES cell culture that does not have gene X D) Neomycin makes the gene X knock-out mice resistant to Candida infection

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Answer & Solution

Answer: Option A

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2

The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to

A) Remove large proteins B) Remove collagen only C) Remove mycoplasma and microorganisms D) Remove foaming agents

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Answer & Solution

Answer: Option C

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3

During the media preparation for cultivation of cells, insoluble precipitates of calcium phosphates are often formed, identify which method can be adopted to avoid this problem?

A) Hold the pH at 5.6 B) Hold the pH at 7.5 C) Add calcium salt first and then phosphate source D) None of the above

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Answer & Solution

Answer: Option D

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4

To create a transgenic organism:

A) Introduce foreign DNA into a gamete or fertilized ovum B) Inject a gene of interest into a somatic cell C) Inject a gene of interest into several somatic cells D) Introduce foreign DNA into somatic cells in culture and transplant them

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Answer & Solution

Answer: Option A

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5

When scientists make an animal superior by view of genotype, introducing some foreign genes in it, the phenomenon is called

A) Tissue culture B) Biotechnology C) Genetic engineering D) Immunisation

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Answer & Solution

Answer: Option C

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6

The following culture systems are used for growing large amount of anchorage dependent animal cells except

A) Roller bottle B) Airlift fermenter C) Hollow fiber reactor D) Microcarriers

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Answer & Solution

Answer: Option B

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7

Which of the following promotes glucose and amino acid uptake by muscle?

A) Adrenaline B) Insulin C) Glucagon D) Cortisol

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Answer & Solution

Answer: Option B

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8

Mitochondrial DNA is replicated from

A) A single ori site bidirectionally B) Two different ori sites in the same direction C) Two different ori sites at different times in opposite directions D) Many sites bidirectionally, like nuclear chromosomes

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Answer & Solution

Answer: Option C

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9

In transgenics, alteration in the sequence of nucleotide in genes are due to
I. Subsitution
II. Deletion
II. Insertion
IV. Rearrangement

A) I and II B) I, II and III C) II and III D) III and IV

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Answer & Solution

Answer: Option B

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10

The sarcoplasmic reticulum in muscle cells acts as a

A) Store of digestive enzymes B) Store of sodium ions C) Store of lipid D) Store of calcium ions

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Answer & Solution

Answer: Option D

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Animal Biotechnology MCQ Questions & Answers | Biotechnology