Increase or decrease in . . . . . . . . leads to protonation or deprotonation of bases which leads to melting of DNA in turn isolating the enzyme after centrifugation.
A. Temperature
B. Ionic strength
C. pH
D. Detergent use
Answer: Option C
Related Questions on Enzyme Engineering in Biotechnology
Which of the following is the best method for isolating enzymes from cell free extract?
A. pH treatment
B. Temperature treatment
C. Chemical treatment
D. Osmotic shock
Which of the following enzyme is used to join two DNA molecule
A. Nuclease
B. Restriction enzymes
C. Lyases
D. Ligases

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