Lysine is being produced in a lab-scale reactor by a threonine auxotroph. After 2 weeks of operation it was observed that the concentration of lysine in the reactor was gradually decreasing. Microbiological assays of reactor samples showed absence of contamination and recorded data showed no change in the operating conditions. The most probable reason for decrease in lysine concentration may be attributed to
A. Accumulation of ethanol
B. Growth of revertants
C. Production of citric acid
D. Unutilized phosphoenol pyruvate
Answer: Option B
This Question Belongs to Biotechnology Engineering >> Bioprocess Engineering And Process Biotechnology
Which of the following techniques is best suited for immobilizing an affinity ligand?
A. Physical adsorption
B. Gel entrapment
C. Crosslinking with a polymer
D. Covalent linkage to a spacer arm
Which one of the following can not be a limiting substrate if Monod's growth kinetics is applicable?
A. Extracellular carbon source
B. Extracellular nitrogen source
C. Dissolved oxygen
D. Intracellular carbon source
A. P-2, Q-3, R-1, S-4
B. P-1, Q-2, R-4, S-3
C. P-3, Q-2, R-4, S-1
D. P-3, Q-4, R-2, S-1
A. 10 h
B. 0.2 h
C. 4.6 h
D. None of the above
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