Match the entries in the Group I with the elution conditions in Group II.
Group I | Group II |
P. Ion exchange chromatography | 1. Isocratic solvent |
Q. Hydrophobic column chromatography | 2. Ampholytes |
R. Gel filtration chromatography | 3. Increasing gradient of salt |
S. Chromatofocusing | 4. Decreasing gradient of polarity |
A. P-4, Q-1, R-2, S-3
B. P-4, Q-3, R-1, S-2
C. P-3, Q-4, R-1, S-2
D. P-3, Q-4, R-2, S-1
Answer: Option B
Related Questions on Bioprocess Engineering and Process Biotechnology
Which of the following techniques is best suited for immobilizing an affinity ligand?
A. Physical adsorption
B. Gel entrapment
C. Crosslinking with a polymer
D. Covalent linkage to a spacer arm
Which one of the following can not be a limiting substrate if Monod's growth kinetics is applicable?
A. Extracellular carbon source
B. Extracellular nitrogen source
C. Dissolved oxygen
D. Intracellular carbon source
A. P-2, Q-3, R-1, S-4
B. P-1, Q-2, R-4, S-3
C. P-3, Q-2, R-4, S-1
D. P-3, Q-4, R-2, S-1
A. 10 h
B. 0.2 h
C. 4.6 h
D. None of the above
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