Examveda
Examveda

Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm-1. At a concentration of 50 mg L-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:

A. 21.4 mg cm-3

B. 214 mg cm-3

C. 2.14 mg cm-3

D. None of the above

Answer: Option D


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Related Questions on Bioprocess Engineering and Process Biotechnology

During cultivation of microorganisms in a fermenter, various parameters are controlled by appropriate sensor (probe). Match each probe in Group I with the appropriate response mechanism in Group II.

Group I (Probe) Group II (Response)
P. Thermistor 1. Activation of acid/alkali pump
Q. Oxygen electrode 2. Activation of vegetable oil pump
R. Metal rod 3. Activation of hot/cold water pump
S. pH electrode 4. Increase/decrease in stirrer motor speed

A. P-2, Q-3, R-1, S-4

B. P-1, Q-2, R-4, S-3

C. P-3, Q-2, R-4, S-1

D. P-3, Q-4, R-2, S-1