Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm-1. At a concentration of 50 mg L-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:
A. 21.4 mg cm-3
B. 214 mg cm-3
C. 2.14 mg cm-3
D. None of the above
Answer: Option D
Related Questions on Bioprocess Engineering and Process Biotechnology
Which of the following techniques is best suited for immobilizing an affinity ligand?
A. Physical adsorption
B. Gel entrapment
C. Crosslinking with a polymer
D. Covalent linkage to a spacer arm
Which one of the following can not be a limiting substrate if Monod's growth kinetics is applicable?
A. Extracellular carbon source
B. Extracellular nitrogen source
C. Dissolved oxygen
D. Intracellular carbon source
A. P-2, Q-3, R-1, S-4
B. P-1, Q-2, R-4, S-3
C. P-3, Q-2, R-4, S-1
D. P-3, Q-4, R-2, S-1
A. 10 h
B. 0.2 h
C. 4.6 h
D. None of the above
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