The DNA sequence shown below is to be amplified by PCR:
5' GCTAAGATCTGAATTTTCC . . . . . . . .
TTGGGCAATAATGTAGCGC 3'
3' CGATTCTAGACTTAAAAGG . . . . . . . .
AACCCGTTATTACATCGCG 5'
Which one of the following pair of primers can be used for this amplification?
A. 5' GGAAATTCAGATCTTAGT 3' and 5' TGGGCAATAATGTAGCGC 3'
B. 5' GCTAAGATCTGAATTTTC 3' and 5' GCGCTACATTATTGCCCA 3'
C. 5' CGGAAATTCAGATCTTAG 3' and 5' GCGCTACATTATTGCCCA 3'
D. 5' GCTAAGATCTGAATTTTC 3' and 5' TGGGCAATAATGTAGCGC 3'
Answer: Option B
This Question Belongs to Biotechnology Engineering >> Molecular Tools In Biotechnology
Expression of hundreds of different genes in DNA microarray technology is monitored by using
A. Radioactive probe
B. Visible chromogenic probe
C. UV absorbing probe
D. Fluorescent probe
A. Transcriptionally controlled
B. Post-transcriptionally controlled
C. Translationally controlled
D. Post-translationally controlled
Which one of the following techniques/tools is not used for inserting a foreign gene into a cell?
A. DNA microarray
B. Electroporation
C. Gene gun
D. Microinjection
A. Polymerase chain reaction
B. DNA microarray
C. Northern hybridization
D. Southern hybridization
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