The enzyme that adds mononucleotide triphosphates to the 3' OH group of a DNA fragment is
A. Polynucleotide kinase
B. Terminal nucleotidyl transferase
C. Terminal phosphoryl transferase
D. All of these
Answer: Option B
A. Polynucleotide kinase
B. Terminal nucleotidyl transferase
C. Terminal phosphoryl transferase
D. All of these
Answer: Option B
Which of the following is the best method for isolating enzymes from cell free extract?
A. pH treatment
B. Temperature treatment
C. Chemical treatment
D. Osmotic shock
Which of the following enzyme is used to join two DNA molecule
A. Nuclease
B. Restriction enzymes
C. Lyases
D. Ligases
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