The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?

Which of the following would not possible to address using a Northern Blot?

A. Location of restriction sites in a particular gene

B. Spatial expression of a particular gene

C. Temporal expression of a particular gene

D. mRNA size

In southern blotting . . . . . . . . is separated by gel electrophoresis

A. DNA

B. m-RNA

C. t-RNA

D. Protein

A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be

A. 4096 bp

B. 256 bp

C. 16 bp

D. 436 bp

Terminal transferase is used

A. To add base at the 3' end of the DNA

B. To add base at the 5' end of the DNA

C. To carry out nick translation

D. To transfer phosphate at the 3' end of the DNA