1. Which of the following would not possible to address using a Northern Blot? A. Location of restriction sites in a particular gene B. Spatial expression of a particular gene C. Temporal expression of a particular gene D. mRNA size Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option A No explanation is given for this question Let's Discuss on Board
2. In southern blotting . . . . . . . . is separated by gel electrophoresis A. DNA B. m-RNA C. t-RNA D. Protein Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option A No explanation is given for this question Let's Discuss on Board
3. A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be A. 4096 bp B. 256 bp C. 16 bp D. 436 bp Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option B No explanation is given for this question Let's Discuss on Board
4. Terminal transferase is used A. To add base at the 3' end of the DNA B. To add base at the 5' end of the DNA C. To carry out nick translation D. To transfer phosphate at the 3' end of the DNA Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option A No explanation is given for this question Let's Discuss on Board
5. Mung bean nuclease could be used for A. DNA synthesis B. Nucleotide hydrolysis C. Trimming single stranded regions in DNA D. Removal of phosphate groups from the ends of the DNA Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option C No explanation is given for this question Let's Discuss on Board
6. Many plasmids have Ampicillin marker. This implies A. The plasmids contain genes for ampicillin biosynthesis B. Ampicillin is required for bacterial growth after transformation C. The plasmid contains the gene encoding β-lactamase D. Ampicillin is essential for cell survival Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option C No explanation is given for this question Let's Discuss on Board
7. DNA synthesis can be measured by estimating incorporation of radio-labelled A. Uracil B. Ribose sugar C. Thymidine D. Adenine Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option C No explanation is given for this question Let's Discuss on Board
8. In agarose gel electrophoresis A. DNA migrates towards the negative electrode B. Supercoiled plasmids migrate slower than their nicked counterparts C. Larger molecules migrate faster than smaller molecules D. Ethidium bromide can be used to visualize the DNA Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option D No explanation is given for this question Let's Discuss on Board
9. A cloning vector consisting of CoS site inserted in a plasmid, used to clone DNA fragments of λ phage is A. Phage mid B. Cosmid C. Plasmid D. YAC Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option B No explanation is given for this question Let's Discuss on Board
10. Northern blotting is developed by A. Ed Southern B. Thomas et al C. Alwine et al D. E. Northern Answer & Solution Discuss in Board Save for Later Answer & Solution Answer: Option C No explanation is given for this question Let's Discuss on Board
