Methods and Techniques in Applied Biology MCQ Questions & Answers

Methods and Techniques in Applied Biology MCQ Questions & Answers | Biotechnology

Learn and Practice Methods and Techniques in Applied Biology MCQ Questions & Answers Available for Biotechnology Engineering Students to Clear GATE Exams, Technical Interviews, Competitive Examinations, and Other Entrance Exams. The Methods and Techniques in Applied Biology Chapter is Especially Important for Biotechnology Engineering and GATE Students.

Which of the following would not possible to address using a Northern Blot?

A) Location of restriction sites in a particular gene B) Spatial expression of a particular gene C) Temporal expression of a particular gene D) mRNA size

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Answer & Solution

Answer: Option A

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In southern blotting . . . . . . . . is separated by gel electrophoresis

A) DNA B) m-RNA C) t-RNA D) Protein

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Answer & Solution

Answer: Option A

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A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be

A) 4096 bp B) 256 bp C) 16 bp D) 436 bp

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Answer & Solution

Answer: Option B

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Terminal transferase is used

A) To add base at the 3' end of the DNA B) To add base at the 5' end of the DNA C) To carry out nick translation D) To transfer phosphate at the 3' end of the DNA

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Answer & Solution

Answer: Option A

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Mung bean nuclease could be used for

A) DNA synthesis B) Nucleotide hydrolysis C) Trimming single stranded regions in DNA D) Removal of phosphate groups from the ends of the DNA

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Answer & Solution

Answer: Option C

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Many plasmids have Ampicillin marker. This implies

A) The plasmids contain genes for ampicillin biosynthesis B) Ampicillin is required for bacterial growth after transformation C) The plasmid contains the gene encoding β-lactamase D) Ampicillin is essential for cell survival

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Answer & Solution

Answer: Option C

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DNA synthesis can be measured by estimating incorporation of radio-labelled

A) Uracil B) Ribose sugar C) Thymidine D) Adenine

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Answer & Solution

Answer: Option C

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In agarose gel electrophoresis

A) DNA migrates towards the negative electrode B) Supercoiled plasmids migrate slower than their nicked counterparts C) Larger molecules migrate faster than smaller molecules D) Ethidium bromide can be used to visualize the DNA

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Answer & Solution

Answer: Option D

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A cloning vector consisting of CoS site inserted in a plasmid, used to clone DNA fragments of λ phage is

A) Phage mid B) Cosmid C) Plasmid D) YAC

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Answer & Solution

Answer: Option B

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Northern blotting is developed by

A) Ed Southern B) Thomas et al C) Alwine et al D) E. Northern

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Answer & Solution

Answer: Option C

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