The schematic of a plasmid with a gap in one of the strands is shown below:
Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?
P. DNA ligase
Q. Alkaline phosphatase
R. DNA polymerase
S. Polynucleotide kinase
A. P only
B. P, R and S only
C. P and R only
D. P, Q and R only
Answer: Option B
A. No recombinant molecule will ever be detected
B. Both linear and circular molecules will replicate equally well
C. None of the plasmids will express the antibiotic resistance gene located on the plasmid
D. The circular molecules will be amplified by the cells
A. Addition of lactose to the medium
B. Removal of all glucose from the medium
C. Addition of excess glucose to the medium
D. Addition of allo-lactose to the medium
A. Both [A] and [R] are true and [R] is the correct reason for [A]
B. Both [A] and [R] are true but [R] is not the correct reason for [A]
C. Both [A] and [R] are false
D. [A] is false but [R] is true
A. P only
B. P, R and S only
C. P and R only
D. P, Q and R only
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