There are two different ways of determining the nucleotide sequence of...

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There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is

A. The differential interaction of the bases with particular dyes

B. Extension of a synthetic primer and reliable termination of DNA repair synthesis

C. The correlation of restriction sites with the end-label of the DNA

D. The ability to 'sequence' both strands of the DNA duplex simultaneously

Answer: Option D

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