Two monomeric His-tagged proteins of identical molecular weight are present in...

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Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?

A. Denaturing polyacrylamide gel electrophoresis

B. Size-exclusion chromatography

C. Ion-exchange chromatography

D. Nickel affinity chromatography

Answer: Option C


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