Examveda

Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?

A. Denaturing polyacrylamide gel electrophoresis

B. Size-exclusion chromatography

C. Ion-exchange chromatography

D. Nickel affinity chromatography

Answer: Option C

Join The Discussion

Related Questions on Bioprocess Engineering and Process Biotechnology

A. Physical adsorption

B. Gel entrapment

C. Crosslinking with a polymer

D. Covalent linkage to a spacer arm

View Answer

A. Extracellular carbon source

B. Extracellular nitrogen source

C. Dissolved oxygen

D. Intracellular carbon source

View Answer

Group I (Probe)	Group II (Response)
P. Thermistor	1. Activation of acid/alkali pump
Q. Oxygen electrode	2. Activation of vegetable oil pump
R. Metal rod	3. Activation of hot/cold water pump
S. pH electrode	4. Increase/decrease in stirrer motor speed

A. P-2, Q-3, R-1, S-4

B. P-1, Q-2, R-4, S-3

C. P-3, Q-2, R-4, S-1

D. P-3, Q-4, R-2, S-1

View Answer

A. 10 h

B. 0.2 h

C. 4.6 h

D. None of the above

View Answer