Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately

Which of the following would not possible to address using a Northern Blot?

A. Location of restriction sites in a particular gene

B. Spatial expression of a particular gene

C. Temporal expression of a particular gene

D. mRNA size

In southern blotting . . . . . . . . is separated by gel electrophoresis

A. DNA

B. m-RNA

C. t-RNA

D. Protein

A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be

A. 4096 bp

B. 256 bp

C. 16 bp

D. 436 bp

Terminal transferase is used

A. To add base at the 3' end of the DNA

B. To add base at the 5' end of the DNA

C. To carry out nick translation

D. To transfer phosphate at the 3' end of the DNA