What are the experimental steps needed for screening an expression library for clone encoding a protein X that has been isolated and purified?
P. mRNA isolation
Q. Antibody preparation
R. Cloning into an appropriate vector
S. Western blotting on transferred plaques
A. P, S
B. Q, S
C. Q, R
D. R, S
Answer: Option C
Related Questions on Recombinant DNA Technology
A. No recombinant molecule will ever be detected
B. Both linear and circular molecules will replicate equally well
C. None of the plasmids will express the antibiotic resistance gene located on the plasmid
D. The circular molecules will be amplified by the cells
A. Addition of lactose to the medium
B. Removal of all glucose from the medium
C. Addition of excess glucose to the medium
D. Addition of allo-lactose to the medium
A. Both [A] and [R] are true and [R] is the correct reason for [A]
B. Both [A] and [R] are true but [R] is not the correct reason for [A]
C. Both [A] and [R] are false
D. [A] is false but [R] is true
A. P only
B. P, R and S only
C. P and R only
D. P, Q and R only
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