Which of the following is incorrect match?

Solution(By Examveda Team)

A. Diene's stain - Mycoplasma
It involves the use of Diene's stain, which is widely used to detect animal mycoplasmas and plant spiroplasmas in culture. Diene's stain was prepared by dissolving 2.5 g of methylene blue, 1.25 g of azure II, 10.0 g of maltose, and 0.25 g of sodium carbonate in 100 ml distilled water.

B. Fontana stain - Spirochaetes
Masson-Fontana silver stain provides direct evidence of spirochaetes and hence can be used as a supplemental or confirmatory test for primary syphilis.

C. Machiavello stain - Chlamydia
The Macchiavello technique is very widely used in rickettsia, especially for infected yolk sacs, and chlamydia even though the colour differentiation of the rickettsiae is undependable and usually of low contrast.

D. Acid fast stain - Staphylococci is not correctly matched.
Staphyloccocus aureus is non-acid-fast bacteria, which upon treatment with acid-alcohol solvent, loses the primary red colour, due to the absence of mycolic acid in gf `its cell wall. This step is followed by the addition of a counterstain, methylene blue. This stains Staphyloccocus aureus blue.