Which of the following statements gives a correct explanation for the use of vectors containing drug resistance genes in the cloning of recombinant DNA (cDNA) molecules?
A. The products of the drug resistance genes protect the cDNA from destruction by the host cells
B. The drug resistance genes provide additional base sequences that enable the vector to accommodate larger inserts of cDNA
C. Entry of the vector containing the cDNA and the drug resistance genes into the host cell renders the latter identifiable as it is now resistant to antibiotic drugs
D. The cloned cDNA imparts drug resistance upon any cellular system with which it is used
Answer: Option C
This Question Belongs to Biotechnology Engineering >> Methods And Techniques In Applied Biology
Which of the following would not possible to address using a Northern Blot?
A. Location of restriction sites in a particular gene
B. Spatial expression of a particular gene
C. Temporal expression of a particular gene
D. mRNA size
In southern blotting . . . . . . . . is separated by gel electrophoresis
A. DNA
B. m-RNA
C. t-RNA
D. Protein
A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be
A. 4096 bp
B. 256 bp
C. 16 bp
D. 436 bp
A. To add base at the 3' end of the DNA
B. To add base at the 5' end of the DNA
C. To carry out nick translation
D. To transfer phosphate at the 3' end of the DNA
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