Which statement correctly describes sequential steps in cDNA cloning?
A. Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector
B. mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase, ligation to vector
C. mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector
D. Double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector
Answer: Option A

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