Which statement correctly describes sequential steps in cDNA cloning?
A. Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector
B. mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase, ligation to vector
C. mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector
D. Double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector
Answer: Option A
This Question Belongs to Biotechnology Engineering >> Methods And Techniques In Applied Biology
Which of the following would not possible to address using a Northern Blot?
A. Location of restriction sites in a particular gene
B. Spatial expression of a particular gene
C. Temporal expression of a particular gene
D. mRNA size
In southern blotting . . . . . . . . is separated by gel electrophoresis
A. DNA
B. m-RNA
C. t-RNA
D. Protein
A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be
A. 4096 bp
B. 256 bp
C. 16 bp
D. 436 bp
A. To add base at the 3' end of the DNA
B. To add base at the 5' end of the DNA
C. To carry out nick translation
D. To transfer phosphate at the 3' end of the DNA
Join The Discussion