Bioprocess Engineering and Process Biotechnology MCQ Questions & Answers

21

Choose the correct completion of the following statement. A fermenter sterilization in situ is less efficient than continuous heat sterilization because

A) It uses higher temperature for longer time B) It uses longer heating time during which heat is lost C) It uses larger volume and hence takes longer to cool the medium D) It uses steam as the heating source

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Answer & Solution

Answer: Option B

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22

Mechanism of separation of contaminants present in air by fibrous media are

A) Interception B) Inertial impaction C) Diffusion D) All of the above

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Answer & Solution

Answer: Option D

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23

Match the entries in the Group I with the elution conditions in Group II.

Group I	Group II
P. Ion exchange chromatography	1. Isocratic solvent
Q. Hydrophobic column chromatography	2. Ampholytes
R. Gel filtration chromatography	3. Increasing gradient of salt
S. Chromatofocusing	4. Decreasing gradient of polarity

A) P-4, Q-1, R-2, S-3 B) P-4, Q-3, R-1, S-2 C) P-3, Q-4, R-1, S-2 D) P-3, Q-4, R-2, S-1

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Answer & Solution

Answer: Option B

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24

Which of the following cases are likely to lead to faster rates of catalysis by an enzyme immobilized on a negatively charged support?

A) A positively charged substrate and a negatively charged product B) A negatively charged substrate and a positively charged product C) A positively charged substrate and a positively charged product D) None of the above

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Answer & Solution

Answer: Option A

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25

The Pasteur Effect is

A) Inhibition of glucose utilization and lactate accumulation in glycolysis B) Sterilization of milk C) Vaccine production D) Heat treatment of bacteria

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Answer & Solution

Answer: Option A

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26

Fed batch cultivation is suitable for which of the following?
P. Processes with substrate inhibition
Q. Processes with product inhibition
R. High cell density cultivation

A) P and Q only B) P and R only C) Q and R only D) P, Q and R

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Answer & Solution

Answer: Option B

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27

Ultrafiltration process cannot be used for

A) Fractionation of proteins B) Desalting C) Harvesting of cells D) Selective removal of solvent

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Answer & Solution

Answer: Option C

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28

For scaling up for a bioreactor, the following parameter is assumed to be constant

A) Airflow rate B) Diameter of the impeller C) Agitator speed D) Volumetric mass transfer coefficient

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Answer & Solution

Answer: Option D

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29

Which of the following events occur during the stationary phase of bacterial growth?
P. Rise in cell number stops.
Q. Spore formation in some Gram-positive bacteria such as Bacillus subtilis.
R. Cell size increases in some Gram-negative bacteria such as Escherichia coli.
S. Growth rate of bacterial cells nearly equals their death rate.
T. Decrease in peptidoglycan cross-linking.

A) P, Q and S only B) P, S and T only C) Q, R and S only D) P, R and T only

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Answer & Solution

Answer: Option A

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30

In a batch culture of Penicillium chrysogenum, the maximum penicillin synthesis occurs during the

A) Lag phase B) Exponential phase C) Stationary phase D) Death phase

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Answer & Solution

Answer: Option C

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Bioprocess Engineering and Process Biotechnology MCQ Questions & Answers | Biotechnology