Bioprocess Engineering and Process Biotechnology MCQ Questions & Answers

41.
Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, ρ, of a double stranded Cs⁺ DNA is given by the equation ρ = 1.66 + 0.098 X_{G + C}, where X_{G + C} denotes

A. Total number of G and C

B. Mole fraction of G + C

C. Number of GC repeats

D. Ratio of G + C to A + T content

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42.
Match the instruments in Group I with their corresponding measurements in Group II.

Group I Group II

P. Manometer 1. Agitator speed

Q. Rotameter 2. Pressure difference

R. Tachometer 3. Cell number

S. Hemocytometer 4. Air flow rate

A. P-4, Q-1, R-2, S-3

B. P-3, Q-4, R-1, S-2

C. P-2, Q-4, R-1, S-3

D. P-2, Q-1, R-4, S-3

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43.
Autoclaves are routinely used in laboratories for sterilization. They act by

A. Disrupting cell membranes

B. Denaturing proteins

C. Changing physically membrane lipids

D. All of the above

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44.
Determine the correctness or otherwise of the following Assertion (A) and the Reason (R).
Assertion: In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously.
Reason: Synchronous culture could be obtained by starving cells for essential nutrient components.

A. Both (A) and (R) are true and (R) is the correct reason for (A)

B. Both (A) and (R) are true but (R) is not the correct reason for (A)

C. (A) is true but (R) is false

D. (A) is false but (R) is true

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45.
In balanced growth phase of a cell,
P. All components of a cell grow at the same rate.
Q. Specific growth determined by cell number or cell mass would be the same.
R. The growth rate is independent of substrate concentration.
S. The growth rate decreases with decreasing substrate concentration.

A. P, Q and S only

B. Q, R and S only

C. P, Q and R only

D. P only

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46.
Which of the following factors can influence the lag phase of a microbial culture in a batch fermenter?
P. Inoculum size
Q. Inoculum age
R. Medium composition

A. P and Q only

B. Q and R only

C. P and R only

D. P, Q and R

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47.
Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following (given, N = rotations/time, D = diameter of impeller)

A. nND

B. nN²D

C. nND²

D. None of these

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48.
Match the properties in Group I with the downstream operations in Group II.

Group I Group II

P. Size 1. Extraction

Q. Density 2. Distillation

R. Volatility 3. Filtration

S. Solubility 4. Sedimentation

A. P-3, Q-4, R-2, S-1

B. P-4, Q-1, R-2, S-3

C. P-4, Q-3, R-1, S-2

D. P-3, Q-2, R-4, S-1

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49.
The diameters of a large and a small vessel are 1.62 m and 16.2 cm, respectively. The vessels are geometrically similar and operated under similar volumetric agitated power input. The mixing time in the small vessel was found to be 15 s. Determine the mixing time (in seconds) in the large vessel.

A. 15

B. 30

C. 61

D. 122

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50.
Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation?

A. Internal reserves

B. Extracellular substrates

C. Extracellular products

D. Toxic substrates

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Group I	Group II
P. Manometer	1. Agitator speed
Q. Rotameter	2. Pressure difference
R. Tachometer	3. Cell number
S. Hemocytometer	4. Air flow rate

Group I	Group II
P. Size	1. Extraction
Q. Density	2. Distillation
R. Volatility	3. Filtration
S. Solubility	4. Sedimentation

Bioprocess Engineering and Process Biotechnology MCQ Questions & Answers | Biotechnology

41. Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, ρ, of a double stranded Cs+ DNA is given by the equation ρ = 1.66 + 0.098 XG + C, where XG + C denotes

Answer & Solution

42. Match the instruments in Group I with their corresponding measurements in Group II. Group I Group II P. Manometer 1. Agitator speed Q. Rotameter 2. Pressure difference R. Tachometer 3. Cell number S. Hemocytometer 4. Air flow rate

Answer & Solution

43. Autoclaves are routinely used in laboratories for sterilization. They act by

Answer & Solution

Answer & Solution

45. In balanced growth phase of a cell, P. All components of a cell grow at the same rate. Q. Specific growth determined by cell number or cell mass would be the same. R. The growth rate is independent of substrate concentration. S. The growth rate decreases with decreasing substrate concentration.

Answer & Solution

46. Which of the following factors can influence the lag phase of a microbial culture in a batch fermenter? P. Inoculum size Q. Inoculum age R. Medium composition

Answer & Solution

47. Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following (given, N = rotations/time, D = diameter of impeller)

Answer & Solution

48. Match the properties in Group I with the downstream operations in Group II. Group I Group II P. Size 1. Extraction Q. Density 2. Distillation R. Volatility 3. Filtration S. Solubility 4. Sedimentation

Answer & Solution

Answer & Solution

50. Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation?

Answer & Solution

41.
Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, ρ, of a double stranded Cs⁺ DNA is given by the equation ρ = 1.66 + 0.098 X_{G + C}, where X_{G + C} denotes

42.
Match the instruments in Group I with their corresponding measurements in Group II.

Group I Group II

P. Manometer 1. Agitator speed

Q. Rotameter 2. Pressure difference

R. Tachometer 3. Cell number

S. Hemocytometer 4. Air flow rate

43.
Autoclaves are routinely used in laboratories for sterilization. They act by

45.
In balanced growth phase of a cell,
P. All components of a cell grow at the same rate.
Q. Specific growth determined by cell number or cell mass would be the same.
R. The growth rate is independent of substrate concentration.
S. The growth rate decreases with decreasing substrate concentration.

46.
Which of the following factors can influence the lag phase of a microbial culture in a batch fermenter?
P. Inoculum size
Q. Inoculum age
R. Medium composition

47.
Since mammalian cells are sensitive to shear, scale-up of a mammalian cell process must consider, among other parameters, the following (given, N = rotations/time, D = diameter of impeller)

48.
Match the properties in Group I with the downstream operations in Group II.

Group I Group II

P. Size 1. Extraction

Q. Density 2. Distillation

R. Volatility 3. Filtration

S. Solubility 4. Sedimentation

50.
Which one of the following is catabolized during endogenous metabolism in a batch bacterial cultivation?