Bioprocess Engineering and Process Biotechnology MCQ Questions & Answers

51.
Which of the following statements are true about bioreactors?
P. Continuous bioreactors provide less degree of control and uniform product quality than batch bioreactors.
Q. Batch bioreactors are ideally suited for reaction with substrate inhibition.
R. Choice of a bioreactor is dictated by kinetic considerations.
S. Fed batch bioreactors are also called semi-batch bioreactors.

A. P, Q

B. Q, S

C. R, S

D. P, R

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52.
Which of the following statements is always correct about an ideal chemostat?
P. Substrate concentration inside the chemostat is equal to that in the exit stream.
Q. Optimal dilution rate is lower than critical dilution rate.
R. Biomass concentration increases with increase in dilution rate.
S. Cell recirculation facilitates operation beyond critical dilution rate.

A. P and Q only

B. P, R and S only

C. P and S only

D. P, Q and S only

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53.
Which of the following statements applies to the operation of a fed-batch process?

A. The volume of the culture increases with time

B. It helps controlling repressive effects of the nutrient being fed

C. It eliminates the need for oxygen supply

D. Only A and B of the above

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54.
Wash out (as defined by D = μ_max) of a continuous stirred tank fermenter is characterized by (X = biomass, S = substrate concentration in bioreactor, S₀ = substrate concentration in feed, P = product concentration in bioreactor).

A. X = 0, S = 0, P = 0

B. X = 0, S = S₀, P = 0

C. X = 0, S < 0, P = 0

D. X < 0, S < 0, P < 0

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55.
Match each parameter in Group I with the appropriate measuring device in Group II.

Group I Group II

P. Pressure 1. Photometer

Q. Foam 2. Rotameter

R. Turbidity 3. Diaphragm gauge

S. Flow rate 4. Rubber sheathed electrode

A. P-3, Q-4, R-1, S-2

B. P-1, Q-3, R-2, S-4

C. P-4, Q-1, R-2, S-3

D. P-1, Q-2, R-3, S-4

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56.
To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation?

A. Culture agitation to maintain oxygen supply

B. Restriction of the entry of contaminating organisms

C. Control of parameters like pH and temperature

D. Maintenance of constant culture volume

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57.
Determine the correctness or otherwise of the following Assertion (A) and Reason (R).
Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation.
Reason: Immobilization protects the plant cells from shear forces in the bioreactor.

A. Both (A) and (R) are true and (R) is the correct reason for (A)

B. Both (A) and (R) are true but (R) is not the correct reason for (A)

C. (A) is true but (R) is false

D. (A) is false but (R) is true

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58.
Which of the following statements is incorrect about immobilized plant cell cultures?

A. It is possible to use high cell densities

B. Cells remain active for long periods

C. Cell products or inhibitors can be removed easily

D. It provides low shear resistance to cells

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59.
Which one of the following relations holds true for the specific growth rate (μ) of a microorganism in the death phase?

A. μ = 0

B. μ < 0

C. μ = μ_max

D. μ < μ < μ_max

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60.
Match the coefficients in Group I with their corresponding downstream processing Steps given in Group II.

Group I Group II

P. Sedimentation coefficient 1. Aqueous two-phase extraction

Q. Partition coefficient 2. Ultrafiltration

R. Rejection coefficient 3. Dialysis

S. Activity coefficient 4. Centrifugation

A. P-3, Q-1, R-4, S-2

B. P-2, Q-1, R-4, S-3

C. P-4, Q-3, R-1, S-2

D. P-4, Q-1, R-2, S-3

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Group I	Group II
P. Pressure	1. Photometer
Q. Foam	2. Rotameter
R. Turbidity	3. Diaphragm gauge
S. Flow rate	4. Rubber sheathed electrode

Group I	Group II
P. Sedimentation coefficient	1. Aqueous two-phase extraction
Q. Partition coefficient	2. Ultrafiltration
R. Rejection coefficient	3. Dialysis
S. Activity coefficient	4. Centrifugation

Bioprocess Engineering and Process Biotechnology MCQ Questions & Answers | Biotechnology

Answer & Solution

Answer & Solution

53. Which of the following statements applies to the operation of a fed-batch process?

Answer & Solution

54. Wash out (as defined by D = μmax) of a continuous stirred tank fermenter is characterized by (X = biomass, S = substrate concentration in bioreactor, S0 = substrate concentration in feed, P = product concentration in bioreactor).

Answer & Solution

55. Match each parameter in Group I with the appropriate measuring device in Group II. Group I Group II P. Pressure 1. Photometer Q. Foam 2. Rotameter R. Turbidity 3. Diaphragm gauge S. Flow rate 4. Rubber sheathed electrode

Answer & Solution

56. To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation?

Answer & Solution

57. Determine the correctness or otherwise of the following Assertion (A) and Reason (R). Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation. Reason: Immobilization protects the plant cells from shear forces in the bioreactor.

Answer & Solution

58. Which of the following statements is incorrect about immobilized plant cell cultures?

Answer & Solution

59. Which one of the following relations holds true for the specific growth rate (μ) of a microorganism in the death phase?

Answer & Solution

Answer & Solution

53.
Which of the following statements applies to the operation of a fed-batch process?

54.
Wash out (as defined by D = μ_max) of a continuous stirred tank fermenter is characterized by (X = biomass, S = substrate concentration in bioreactor, S₀ = substrate concentration in feed, P = product concentration in bioreactor).

55.
Match each parameter in Group I with the appropriate measuring device in Group II.

Group I Group II

P. Pressure 1. Photometer

Q. Foam 2. Rotameter

R. Turbidity 3. Diaphragm gauge

S. Flow rate 4. Rubber sheathed electrode

56.
To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation?

57.
Determine the correctness or otherwise of the following Assertion (A) and Reason (R).
Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation.
Reason: Immobilization protects the plant cells from shear forces in the bioreactor.

58.
Which of the following statements is incorrect about immobilized plant cell cultures?

59.
Which one of the following relations holds true for the specific growth rate (μ) of a microorganism in the death phase?