Methods and Techniques in Applied Biology MCQ Questions & Answers

31.
Match List-I (cloning vector) with List-II (character) and select the correct answer:

List-I: Cloning vector List-II: Character

a. Yeast episomal plasmid (YEp) 1. Integrates with host chromosome for replication

b. Yeast integrative plasmid (YIp) 2. Carries a chromosomal origin of replication

c. Yeast replicative plasmid (YRp) 3. Carries origin of replication of the 2 mm plasmid

A. a-3, b-2, c-1

B. a-2, b-1, c-3

C. a-3, b-1, c-2

D. a-2, b-3, c-1

Answer & Solution Discuss in Board Save for Later

32.
Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed?

A. In situ hybridization

B. DNA microarray

C. Protein microarray

D. Reporter gene fusion including introns

Answer & Solution Discuss in Board Save for Later

33.
Which of the following are not valid methods of labelling duplex DNA?

A. 5'-end labelling with polynucleotide kinase

B. 3'-end labelling with polynucleotide kinase

C. 3'-end labelling with terminal transferase

D. Nick translation

Answer & Solution Discuss in Board Save for Later

34.
BAC, which can be used to clone large DNA fragments, is derived from

A. ColE plasmid

B. F plasmid

C. 2μ

D. Mu phage

Answer & Solution Discuss in Board Save for Later

35.
Restriction endonucleases hydrolyzes polynucleotide from

A. Only the 5' end

B. From either terminal

C. At an internal phosphodiester bond

D. A phosphodiester bond within a specific sequence

Answer & Solution Discuss in Board Save for Later

36.
ELISA is used to detect viruses where the key reagent is

A. Alkaline phosphatase

B. Catalase

C. DNA probe

D. Rnase

Answer & Solution Discuss in Board Save for Later

37.
Genetically engineered male sterile crop plants may be produced by inserting

A. BT toxin gene

B. Barnase gene

C. Lectin gene

D. Chitinase gene

Answer & Solution Discuss in Board Save for Later

38.
Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately

A. 4 : 8

B. 8 : 4

C. 1 : 64

D. 1 : 26

Answer & Solution Discuss in Board Save for Later

39.
Which of the following is a desirable characteristic for a cloning plasmid?

A. A site at which replication can be initiated

B. One or more unique restriction endonuclease sites

C. One or more antibiotic-resistance or drug resistance genes

D. All of the above

Answer & Solution Discuss in Board Save for Later

40.
Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)?

A. A missense mutation within the protein coding region of a gene

B. A silent mutation within the protein coding region of a gene

C. A single base change within the intron sequence of a gene

D. An error in RNA splicing that mistakenly removes an exon during RNA processing

Answer & Solution Discuss in Board Save for Later

List-I: Cloning vector	List-II: Character
a. Yeast episomal plasmid (YEp)	1. Integrates with host chromosome for replication
b. Yeast integrative plasmid (YIp)	2. Carries a chromosomal origin of replication
c. Yeast replicative plasmid (YRp)	3. Carries origin of replication of the 2 mm plasmid

Methods and Techniques in Applied Biology MCQ Questions & Answers | Biotechnology

Answer & Solution

32. Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed?

Answer & Solution

33. Which of the following are not valid methods of labelling duplex DNA?

Answer & Solution

34. BAC, which can be used to clone large DNA fragments, is derived from

Answer & Solution

35. Restriction endonucleases hydrolyzes polynucleotide from

Answer & Solution

36. ELISA is used to detect viruses where the key reagent is

Answer & Solution

37. Genetically engineered male sterile crop plants may be produced by inserting

Answer & Solution

38. Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately

Answer & Solution

39. Which of the following is a desirable characteristic for a cloning plasmid?

Answer & Solution

40. Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)?

Answer & Solution

32.
Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed?

33.
Which of the following are not valid methods of labelling duplex DNA?

34.
BAC, which can be used to clone large DNA fragments, is derived from

35.
Restriction endonucleases hydrolyzes polynucleotide from

36.
ELISA is used to detect viruses where the key reagent is

37.
Genetically engineered male sterile crop plants may be produced by inserting

38.
Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately

39.
Which of the following is a desirable characteristic for a cloning plasmid?

40.
Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)?