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Methods and Techniques in Applied Biology MCQ Questions & Answers | Biotechnology

Learn and Practice Methods and Techniques in Applied Biology MCQ Questions & Answers Available for Biotechnology Engineering Students to Clear GATE Exams, Technical Interviews, Competitive Examinations, and Other Entrance Exams. The Methods and Techniques in Applied Biology Chapter is Especially Important for Biotechnology Engineering and GATE Students. Page-4 section-1

31
Match List-I (cloning vector) with List-II (character) and select the correct answer:
List-I: Cloning vector List-II: Character
a. Yeast episomal plasmid (YEp) 1. Integrates with host chromosome for replication
b. Yeast integrative plasmid (YIp) 2. Carries a chromosomal origin of replication
c. Yeast replicative plasmid (YRp) 3. Carries origin of replication of the 2 mm plasmid
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Answer & Solution
Answer: Option C
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32
Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed?
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Answer & Solution
Answer: Option B
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33
Which of the following are not valid methods of labelling duplex DNA?
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Answer & Solution
Answer: Option B
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34
BAC, which can be used to clone large DNA fragments, is derived from
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Answer & Solution
Answer: Option B
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35
Restriction endonucleases hydrolyzes polynucleotide from
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Answer: Option D
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36
ELISA is used to detect viruses where the key reagent is
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Answer & Solution
Answer: Option A
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37
Genetically engineered male sterile crop plants may be produced by inserting
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Answer & Solution
Answer: Option A
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38
Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately
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Answer & Solution
Answer: Option D
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39
Which of the following is a desirable characteristic for a cloning plasmid?
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Answer & Solution
Answer: Option D
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40
Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)?
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Answer & Solution
Answer: Option D
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