Methods and Techniques in Applied Biology MCQ Questions & Answers | Biotechnology

Which of the following is incorrect?

A gene that does not contain introns has an Eco RI site just down stream of the translational termination codon in the 3' untranslated region. The size of the gene is 4 Kbp. The length of 3' UTR is 2 Kbp, the 5' UTR is 1 Kbp and that the open reading frame is 1kbp. Upon probing a southern blot of an Eco RI digested genomic DNA with the radioactively labelled fragment containing only the open reading frame, the size of the band detected by hybridization would be

Cells deficient in hypoxanthine guanine phospho-ribosyl transferase (HGPRT)

Which of the following statements gives a correct explanation for the use of vectors containing drug resistance genes in the cloning of recombinant DNA (cDNA) molecules?

There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is

The ability to clone an adult animal from the nucleus of a differentiated somatic cell proves that

For protoplast fusion to be successful in plant cells.

The process used for separation of protein in polyacrylamide gel is called:

The technique which involves addition or deletions of genes is

In Southern blotting, DNA is fixed on