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Molecular Tools in Biotechnology MCQ Questions & Answers | Biotechnology

Learn and Practice Molecular Tools in Biotechnology MCQ Questions & Answers Available for Biotechnology Engineering Students to Clear GATE Exams, Technical Interviews, Competitive Examinations, and Other Entrance Exams. The Molecular Tools in Biotechnology Chapter is Especially Important for Biotechnology Engineering and GATE Students. Page-2 section-1

11
Enzyme used in 'cycle' sequencing of DNA is
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Answer & Solution
Answer: Option D
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12
Which one of the following is not a requirement for a PCR reaction?
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Answer: Option C
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13
Which of the following statements about gene therapy are correct?
P. Affected individuals, but not their progeny, can be cured through germline gene therapy.
Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy.
R. Affected individuals, but not their progeny, can be cured through somatic gene therapy.
S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.
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Answer: Option C
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14
Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction?
P. Mg2+ concentration
Q. pH
R. Annealing temperature
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Answer: Option D
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15
The heterozygosity of any locus can be ascertained by
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Answer: Option B
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16
For PCR to amplify the sequence
ATCTTCTACG . . . . . . . . AAGCPTGCGG
TAGAAGATGC . . . . . . . .TTCGAACGCC
The required primers are
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Answer: Option B
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17
In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be
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Answer: Option B
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18
In human populations 4% of the individuals are homozygous recessive to a specific RFLP marker. What percentage of individuals does you expect to be heterozygous for this trait?
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Answer: Option D
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19
Which of the following fluorescent probes is used to monitor the progress of amplification in Real time PCR?
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Answer: Option A
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20
Match items in Group I with correct options from those given in Group II.
Group I Group II
P. VNTR sequence 1. Gene regulation on the same chromosome
Q. Leader sequence 2. Ribosome binding site
R. SD sequence 3. DNA fingerprinting
S. cis-acting sequence 4. Functions in attenuation
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Answer: Option D
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